thermo二抗A21207|Invitrogen抗體*

我們提供廣泛的Invitrogen™二抗結(jié)合物，具有良好的特異性特征，并使用多種優(yōu)質(zhì)熒光染料進(jìn)行標(biāo)記，包括Invitrogen™Alexa Fluor™熒光染料。 熒光二抗綴合物可用于其特定靶標(biāo)的檢測，分選或純化，是熒光顯微鏡和共聚焦激光掃描顯微鏡，流式細(xì)胞儀和熒光西方檢測的理想選擇。 我們提供的熒光標(biāo)記的廣泛性使我們的試劑可以適應(yīng)幾乎任何熒光檢測系統(tǒng)。

可以以三種形式提供二抗：完整IgG，二價F（ab'）2片段和單價Fab片段。 由于許多免疫球蛋白結(jié)構(gòu)域的結(jié)構(gòu)具有高度保守性，因此大多數(shù)類特異性第二抗體必須經(jīng)親和純化和交叉吸附以實現(xiàn)與其他免疫球蛋白的小交叉反應(yīng)。

我們的二抗結(jié)合物較常通過用來自目標(biāo)物種的匯集的免疫球蛋白群免疫宿主動物來制備，并且可以進(jìn)一步純化和修飾（例如，免疫親和層析，抗體片段化，標(biāo)記綴合等）以產(chǎn)生高度特異性的試劑。。 在*輪純化中，回收與免疫抗體結(jié)合的完整免疫球蛋白，并且主要由~150-kDa IgG類組成。 進(jìn)一步純化，例如，用蛋白A或G，除去與靶特異性免疫球蛋白重鏈和/或輕鏈反應(yīng)的親和純化的抗體，除去所有不需要的免疫球蛋白類別。

thermo二抗A21207|Invitrogen抗體*

Product Specific Information

To minimize cross-reactivity, these donkey anti-rabbit IgG whole antibodies have been affinity-purified and show minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Alexa Fluor染料是目前較值得信賴的熒光染料之一。 Invitrogen™Alexa Fluor 594染料是一種明亮的紅色熒光染料，具有理想的激發(fā)，適用于594 nm激光線。為了在成像和流式細(xì)胞儀中產(chǎn)生穩(wěn)定的信號，Alexa Fluor 594染料在很寬的摩爾范圍內(nèi)對pH不敏感。具有高熒光量子產(chǎn)率和高光穩(wěn)定性的探針允許以高靈敏度檢測低豐度生物結(jié)構(gòu)。 Alexa Fluor 594染料分子可以高摩爾比附著在蛋白質(zhì)上而不會發(fā)生明顯的自猝滅，從而實現(xiàn)更明亮的結(jié)合物和更靈敏的檢測。每種綴合物的標(biāo)記程度通常為每個IgG分子2-8個熒光團分子;每個產(chǎn)品批次的分析證書上都標(biāo)明了確切的標(biāo)簽程度。

使用結(jié)合物溶液：使用前在微量離心機中短暫離心蛋白質(zhì)結(jié)合物溶液;僅將上清液添加到實驗中。該步驟將有助于消除在儲存期間可能形成的任何蛋白質(zhì)聚集體，從而減少非特異性背景染色。由于染色方案隨應(yīng)用而變化，因此應(yīng)根據(jù)經(jīng)驗確定抗體的適當(dāng)稀釋度。對于熒光團標(biāo)記的抗體，對于大多數(shù)免疫組織化學(xué)和流式細(xì)胞術(shù)應(yīng)用，終濃度1-10μg/ mL應(yīng)該是令人滿意的。

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1-10 µg/mL

Immunocytochemistry (ICC)

2 µg/mL

Immunofluorescence (IF)

2 µg/mL

Immunohistochemistry (IHC)

1-10 µg/mL

Published Applications

Immunohistochemistry (IHC)

See 6 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 6 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 5 publications below

Immunocytochemistry (ICC)

See 2 publications below

Immunohistochemistry - Free Floating (IHC (Free))

See 1 publication below

Miscellaneous PubMed (MISC)

See 13 publications below

Product Specifications

Species Reactivity

Rabbit

Host / Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor® 594

Excitation/Emission Profile

View spectra 

Form

liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

RRID

AB_141637

Target

IgG

Antibody Form

Whole Antibody