Jackson|二抗709-005-149*

通過免疫親和層析從抗血清分離全IgG抗體作為完整分子。 它們具有Fc部分和兩個通過二硫鍵連接在一起的抗原結(jié)合Fab部分，因此它們是二價的。 據(jù)報道平均分子量約為160kDa。 整個IgG形式的抗體適用于大多數(shù)免疫檢測程序，并且是成本效益的。

基于免疫電泳和/或ELISA，抗體與全分子人IgG反應。 它還與其他人免疫球蛋白的輕鏈反應。 未檢測到針對非免疫球蛋白血清蛋白的抗體。 已經(jīng)通過ELISA和/或固相吸附測試抗體以確保與牛，雞，山羊，豚鼠，敘利亞倉鼠，馬，小鼠，兔，大鼠和綿羊血清蛋白的小交叉反應，但它可以交叉 -  與來自其他物種的免疫球蛋白反應。

Jackson|二抗709-005-149*

Physical State: Sterile-filtered liquid 
Storage:

Store at 2-8°C under sterile conditions. Prepare working dilution on day of use. 
Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. 
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
Preservative: None 

Suggested Working Concentration or Dilution Range:

10-20 µg / ml 

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.